The generation process of the recombinant human glucose-6-phosphatase (G6PC) involves ligating a gene fragment encoding amino acids 82 to 117 of the human G6PC protein with an N-terminal 6xHis-sumostar-tag. This fusion construct is carefully cloned into an appropriate expression vector. Subsequently, the recombinant vector is introduced into the yeast expression system, enabling the efficient expression of the desired recombinant human G6PC protein. Upon successful expression, the recombinant G6PC protein is extracted and purified from the yeast cell lysate, achieving a purity level of over 90% as confirmed by SDS-PAGE analysis. G6PC, an essential enzyme involved in glucose metabolism, has also been linked to cancer, playing a critical role in regulating glucose homeostasis within cancer cells.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[33735],"class_list":["post-108536","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-g6pc1","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n