{"id":109415,"date":"2025-12-25T13:28:09","date_gmt":"2025-12-25T13:28:09","guid":{"rendered":"https:\/\/advbiomart.sytech.site\/product\/recombinant-human-leucine-rich-repeat-containing-g-protein-coupled-receptor-5-lgr5-tuncated-acp02039\/"},"modified":"2025-12-25T13:28:10","modified_gmt":"2025-12-25T13:28:10","slug":"recombinant-human-leucine-rich-repeat-containing-g-protein-coupled-receptor-5-lgr5-tuncated-acp02039","status":"publish","type":"product","link":"https:\/\/www.abtriva.com\/cn\/product\/recombinant-human-leucine-rich-repeat-containing-g-protein-coupled-receptor-5-lgr5-tuncated-acp02039\/","title":{"rendered":"Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), Truncated"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"

The synthesis of the recombinant plasmid containing the gene encoding the Human LGR5 protein (22-561aa) is the first step to produce the recombinant Human LGR5 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal myc tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human LGR5 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human LGR5 protein, indicating a purity level exceeding 90%.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[4880],"class_list":["post-109415","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-lgr5","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\nRecombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), Truncated - AbTrivia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.abtriva.com\/cn\/product\/recombinant-human-leucine-rich-repeat-containing-g-protein-coupled-receptor-5-lgr5-tuncated-acp02039\/\" \/>\n<meta property=\"og:locale\" content=\"cn_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), Truncated - AbTrivia\" \/>\n<meta property=\"og:description\" content=\"The synthesis of the recombinant plasmid containing the gene encoding the Human LGR5 protein (22-561aa) is the first step to produce the recombinant Human LGR5 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag and C-terminal myc tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human LGR5 protein from the cell lysate. 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