The recombinant human PRKN protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the Baculovirus that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag and C-terminal Myc tag gene to the gene fragment coding for the 1-465aa of the human PRKN protein. After purification, the product is the recombinant human PRKN protein. This recombinant PRKN protein was subjected to the SDS-PAGE determination. Its purity reaches over 85% evaluated by Bandscan software analysis combined with SAS-PAGE. This PRKN protein ran to the molecular weight of about 55 kDa under SDS-PAGE condition.E3 ubiquitin ligase (also called PRKN) protein functions to recruit an E2 ubiquitin-conjugating enzyme. PRKN interact with the target protein and the E2 enzyme. Therefore, it could impart substrate specificity to the E2. In particular, ubiquitination by PRKN mediates the complex biological process like homeostasis, cell trafficking, and DNA repair. Also, the signaling PRKN involved play crucial roles in cell biology, which could be associated with some famously molecular like MDM2 and BRCA1 that play a part in various cancers. Moreover, based on current researches, mutations in PRKN gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[53836],"class_list":["post-109760","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-prkn","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n