In the production of recombinant Human SIRT1 protein, the gene for SIRT1 (E.coli) was cloned into a vector and expressed as SIRT1 protein in E.coli. The plasmids with the copy of SIRT1, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis tag was used in the process. The purity is 90% determined by SDS-PAGE.SIRT1 is a nicotinamide adenine dinucleotide (NAD+)-dependent histone\/circadian deacetylase that comprises 747 residues encompassing a catalytic domain, an extended N- and a C-terminal region. It catalyzes the removal of the acetyl group form various substrates leading to the formation of nicotinamide and O-acetylADP-ribose. NAD+(nicotinamide adenine dinucleotide) plays central roles in energy metabolism as redox carrier. SIRT1, as a NAD dependent protein deacetylase, has been implicated in the pathogenesis of neurodegeneration in neurological diseases including MS. Recent research has identified important signalling functions of NAD+ that involve its consumption. Consumption NAD+ in signaling reactions have been characterized on a molecular level: ADP-ribosyltransferases (ARTs), Sirtuins (SIRTs), and NAD+ glycohydrolases (NADases). Members of these families serve important regulatory functions in various cellular compartments, e.g., by linking the cellular energy state to gene expression in the nucleus, by regulating nitrogen metabolism in mitochondria, and by sensing tissue damage in the extracellular compartment.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[21626],"class_list":["post-109788","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-sirt1","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n