Amino acids 2-218 form the expressed segment for recombinant Human HPRT1. The calculated molecular weight for this HPRT1 protein is 28.4 kDa. This protein is generated in a e.coli-based system. The N-terminal 6xHis tag was fused into the coding gene segment of HPRT1, making it easier to detect and purify the HPRT1 recombinant protein in the later stages of expression and purification.The human hypoxanthine-guanine phosphoribosyltransferase (HPRT1) is an essential enzyme that plays a critical role in the purine salvage pathway, which is responsible for recycling and salvaging purine bases (hypoxanthine and guanine) to synthesize nucleotides. HPRT1 catalyzes the conversion of hypoxanthine and guanine to their respective nucleotides, inosine monophosphate (IMP) and guanosine monophosphate (GMP), utilizing phosphoribosyl pyrophosphate (PRPP) as a substrate. Mutations in the HPRT1 gene can lead to Lesch-Nyhan syndrome, a rare genetic disorder characterized by neurological and behavioral abnormalities, as well as overproduction of uric acid. Research on HPRT1 is crucial for understanding purine metabolism, nucleotide biosynthesis, and the molecular basis of associated genetic disorders.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[70969],"class_list":["post-110150","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-hprt1","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n