Amino acids 1-267 form the expressed segment for recombinant Human PHOSPHO1. The expected molecular weight for the PHOSPHO1 protein is calculated to be 33.7 kDa. Expression of this PHOSPHO1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the PHOSPHO1 encoding gene fragment was conducted, allowing for easier detection and purification of the PHOSPHO1 protein in subsequent stages.The human phosphoethanolamine\/phosphocholine phosphatase (PHOSPHO1) primarily functions in regulating skeletal mineralization by hydrolyzing phosphocholine and phosphoethanolamine. It is involved in the initiation of hydroxyapatite crystal formation during bone development. In bone and mineralization research, studying PHOSPHO1 contributes to understanding the molecular mechanisms involved in bone mineralization and may have implications for bone-related disorders. PHOSPHO1’s involvement in mineralization processes makes it relevant in dental research as well. Investigating PHOSPHO1 provides insights into skeletal biology, mineral homeostasis, and potential applications in developing therapeutic strategies for skeletal disorders, enhancing the understanding of mineralization processes in different physiological and pathological contexts.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[14272],"class_list":["post-111028","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-phospho1","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n