{"version":"1.0","provider_name":"AbTrivia","provider_url":"https:\/\/www.abtriva.com\/es","author_name":"Sytech","author_url":"https:\/\/www.abtriva.com\/es\/author\/sytech\/","title":"Recombinant Human Prothrombin (F2) - AbTrivia","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"LjTUSSksAK\"><a href=\"https:\/\/www.abtriva.com\/es\/product\/recombinant-human-prothrombin-f2-acp04732\/\">Recombinant Human Prothrombin (F2)<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/www.abtriva.com\/es\/product\/recombinant-human-prothrombin-f2-acp04732\/embed\/#?secret=LjTUSSksAK\" width=\"600\" height=\"338\" title=\"&#8220;Recombinant Human Prothrombin (F2)&#8221; &#8212; AbTrivia\" data-secret=\"LjTUSSksAK\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/www.abtriva.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","description":"The process of expressing the recombinant human F2 protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 44-622aa of the human F2 protein and N-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-tagged recombinant F2 protein was obtained. This recombinant F2 protein is characterized by high purity (>90%, SDS-PAGE). This F2 protein ran along the gel to the band of approximately 66-105 kDa molecular weight.F2 is a gene providing instruction of making a protein named prothrombin (also known as coagulation factor II) in human and belongs to peptidase S1 family. This protein can be cleaved into 4 chains, including activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. It is the precursor of thrombin, which acts as a serine protease cleaving bonds after Arg and Lys and converting soluble fibrinogen into insoluble strands of fibrin and activating factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C."}