The fusion tag N-terminal 10xHis-SUMO tag and C-terminal Myc tag gene was added to the gene sequence corresponding to the E.coli of the human NEFH protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transformed into the E.coli for expression. Following purification, the product is the recombinant human NEFH protein carrying N-terminal 10xHis-SUMO tag and C-terminal Myc tag. The SDS-PAGE assessed the purity of this recombinant NEFH protein up to 85%. It had an apparent molecular weight of approximately 60 kDa. This recombinant NEFH protein may be used in neuroscience research.NEFH is a gene encoding a protein named neurofilament heavy polypeptide (abbreviated NEFH) in human and belongs to intermediate filament family. This protein combines with medium and light subunits to make neurofilaments, which form the framework for nerve cells. Several studies have indicated that NEFH is expressed in podocytes during the disease course and that it prevents the reduction in synaptopodin expression and detachment of podocytes. A novel missense pathogenic variant in NEFH causing rare Charcot-Marie-Tooth neuropathy type 2CC. Currently, reported diseases associated with NEFH include Charcot-Marie-Tooth Disease, Axonal, Type 2Cc and Amyotrophic Lateral Sclerosis.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[587],"class_list":["post-110978","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-nefh","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n