A cDNA sequence coding for full-length human cyclic AMP-dependent transcription factor ATF2 was inserted a 6xHis-SUMO-tag at the N-terminus and then expressed in E.coli. The resulting protein consists of 1-505aa of human ATF2 and has a calculated molecular weight of 70.5 kDa. Its purity is over 90% determined by SDS-PAGE. Under reducing conditions, the gel presented a molecular mass band of about 73 kDa. Glycosylation contributes to the slightly higher molecular mass. This recombinant ATF2 protein may be used for specific antibody production or in the studies of ATF2-related transcription.
\nActivating transcription factor 2 (ATF2) is a member of the activating protein-1 (AP1) transcription factor family that regulates gene expression through homo- or hetero-dimerization with other AP1 transcription factors. ATF2 is also a vital mediator of mammalian cell responses to various stimuli, including stress. Alteration of ATF2 expression and localization has also been associated with the pathology, progression, and chemoresistance of extramammary Paget’s disease, as well as in prostate and head and neck squamous cancers.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[49521],"class_list":["post-111509","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-atf2","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n