A truncated version of Vascular cell adhesion protein 1 (VCAM1) encoding the extracellular domain and carrying a 6xHis-tag at the N-terminus was produced in the yeast. The resulting protein is a partial-length VCAM1 protein. It contains aa of 25-698 of the human VCAM1 protein. It was purified by SDS-PAGE and reached up to 90% in purity. On the reducing SDS-PAEG gel, this VCAM1 protein migrated on the molecular weight band of about 66-116 kDa. In addition to producing specific antibodies, the recombinant VCAM1 protein could also be used in the studies of VACM-related immunology or signal transduction.
\nVCAM-1, or called CD106, is a glycoprotein primarily expressed in endothelial cells. Pro-inflammatory cytokines, ROS, high glucose concentration, oxidized low-density lipoprotein, shear stress, and toll-like receptor agonists, can induce the expression of VCAM-1. VCAM-1 participates in the modulation of inflammation-associated vascular adhesion and the transendothelial migration of leukocytes. Recent studies show the close link of VCAM-1 to the progression of various immunological disorders, such as asthma, rheumatoid arthritis, transplant rejection, and cancer.<\/p>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[168834,18],"product_tag":[44700],"class_list":["post-112090","product","type-product","status-publish","product_cat-proteins","product_cat-recombinant-proteins","product_tag-vcam1","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"\n