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The preparation of the recombinant Human MORC4 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (29-486aa) of foreign DNA is fused to an expression vector and then introduced into e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant targeted protein is subjected to affinity purification. Its purity exceeds 90% as assessed by SDS-PAGE.
The preparation of the recombinant Human MORC4 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (29-486aa) of foreign DNA is fused to an expression vector and then introduced into e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant targeted protein is subjected to affinity purification. Its purity exceeds 90% as assessed by SDS-PAGE.
| Cat.No | ACP01050 | Target Name | MORC4 |
|---|---|---|---|
| Target Synonyms | (Zinc finger CW-type coiled-coil domain protein 2)(Zinc finger CW-type domain protein 4) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 29-486aa |
| Mol Weight | 57.2 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8TE76 |
|---|
Uniprot Id
Q8TE76
Target Species
Human
Target Name
MORC4
Target Full Name
MORC family CW-type zinc finger protein 4
Target Function
Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3. The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0.
Target Subcellular Location
Nucleus.
Target Tissue Specificity
Expressed at low levels in normal tissues, with highest expression levels in placenta and testis. Expression is significantly increased in subset of diffuse large B-cell lymphomas.
Target Research Area
Others
Target Synonyms
dJ75H8.2; MORC family CW type zinc finger 4; MORC family CW-type zinc finger protein 4; Morc4; MORC4_HUMAN; RP1-75H8.4; ZCW4; ZCWCC2; Zinc finger CW type with coiled coil domain 2; Zinc finger CW-type coiled-coil domain protein 2
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