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The production of the recombinant Human SLC34A2 protein begins with the creation of the recombinant plasmid, which is synthesized by inserting the gene encoding the Human SLC34A2 protein (574-689aa) into a plasmid vector. The recombinant plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected and then cultured under conditions conducive to the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-B2M tag. Following expression, the recombinant SLC34A2 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human SLC34A2 protein, demonstrating a purity exceeding 85%.
The production of the recombinant Human SLC34A2 protein begins with the creation of the recombinant plasmid, which is synthesized by inserting the gene encoding the Human SLC34A2 protein (574-689aa) into a plasmid vector. The recombinant plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected and then cultured under conditions conducive to the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-B2M tag. Following expression, the recombinant SLC34A2 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Human SLC34A2 protein, demonstrating a purity exceeding 85%.
| Cat.No | ACP03094 | Target Name | SLC34A2 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 574-689aa | Mol Weight | 27.1 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O95436 |
|---|
Uniprot Id
O95436
Target Species
Human
Target Name
SLC34A2
Target Full Name
Sodium-dependent phosphate transport protein 2B
Target Function
May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.
Target Involvement
Pulmonary alveolar microlithiasis (PALM)
Target Subcellular Location
Membrane; Multi-pass membrane protein.
Target Protein Families
SLC34A transporter family
Target Tissue Specificity
Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium.
Target Research Area
Signal Transduction
Target Synonyms
SLC34A2; Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na(+)-dependent phosphate cotransporter 2B; NaPi3b; Sodium/phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2
Target Background
The protein encoded by this gene is a pH-sensitive sodium-dependent phosphate transporter. Phosphate uptake is increased at lower pH. Defects in this gene are a cause of pulmonary alveolar microlithiasis. Three transcript variants encoding two different isoforms have been found for this gene.
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