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Recombination of a plasmid encoding the Human USP14 protein (1-494aa) is the first step during the production the recombinant Human USP14 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 6xHis tag. After expression, affinity purification is used to isolate and purify the recombinant Human USP14 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human USP14 protein. Its purity exceeds 90%.
Recombination of a plasmid encoding the Human USP14 protein (1-494aa) is the first step during the production the recombinant Human USP14 protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal 6xHis tag. After expression, affinity purification is used to isolate and purify the recombinant Human USP14 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human USP14 protein. Its purity exceeds 90%.
| Cat.No | ACP04225 | Target Name | USP14 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-494aa | Mol Weight | 60.1kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P54578 |
|---|
Uniprot Id
P54578
Target Species
Human
Target Name
USP14
Target Full Name
Ubiquitin carboxyl-terminal hydrolase 14
Target Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
Target Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Target Protein Families
Peptidase C19 family, USP14/UBP6 subfamily
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
Deubiquitinating enzyme 14; TGT; tRNA guanine transglycosylase 60 kD subunit; tRNA guanine transglycosylase; Ubiquitin carboxyl terminal hydrolase 14; Ubiquitin carboxyl-terminal hydrolase 14; Ubiquitin specific peptidase 14; Ubiquitin specific processing protease 14; Ubiquitin specific protease 14; Ubiquitin thiolesterase 14; Ubiquitin-specific-processing protease 14; UBP14_HUMAN; USP 14; USP14
Target Background
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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