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The antibody against mazF was raised in rabbit using the Recombinant Escherichia coli O157:H7 mazF protein (1-111aa ) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
The antibody against mazF was raised in rabbit using the Recombinant Escherichia coli O157:H7 mazF protein (1-111aa ) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in Recombinant protein | All lanes: mazF antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 29 kDa | Observed band size: 29 kDa
| Cat.No | ADC-55358A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | mazF |
| Target Synonyms | Endoribonuclease MazF (EC 3.1.27.-) (Toxin MazF) (mRNA interferase MazF) mazF chpA Z4097 ECs3642 | Form | Liquid |
| Species Reactivity | Escherichia coli O157:H7 | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Escherichia coli O157:H7 mazF protein (1-111aa ) | Target Species | Escherichia coli O157:H7 |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P0AE71 |
Uniprot Id
P0AE71
Target Species
E.coli
Target Name
MAZF
Target Function
Toxic component of a type II toxin-antitoxin (TA) system. MazF is a sequence-specific endoribonuclease that inhibits protein synthesis and induces bacterial stasis. It is very stable, single-strand specific and cleavage is independent of the ribosome. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription.
Target Protein Families
PemK/MazF family
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