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The antibody against FLC was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 60-196 of arabidopsis thaliana FLC (NP_196576.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against FLC was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 60-196 of arabidopsis thaliana FLC (NP_196576.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
| Cat.No | ADA-07922A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | FLC |
| Target Synonyms | AGAMOUS-like 25; AGL25; FLF; FLOWERING LOCUS C; FLOWERING LOCUS F; MADS BOX PROTEIN FLOWERING LOCUS F; REDUCED STEM BRANCHING 6; RSB6; T31P16.130; T31P16_130; FLC | Form | Liquid |
| Species Reactivity | Arabidopsis thaliana | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Before inflorescence, Inflorescences, Rosette leaf, Seedling | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 60-196 of arabidopsis thaliana FLC (NP_196576.1). | Immunogen Sequence | GDNLVKILDRYGKQHADDLKALDHQSKALNYGSHYELLELVDSKLVGSNVKNVSIDALVQLEEHLETALSVTRAKKTELMLKLVENLKEKEKMLKEENQVLASQMENNHHVGAEAEMEMSPAGQISDNLPVTLPLLN |
|---|---|---|---|
| Uniprot ID | Q9S7Q7 |
Uniprot Id
Q9S7Q7
Target Synonyms
AGAMOUS-like 25; AGL25; FLF; FLOWERING LOCUS C; FLOWERING LOCUS F; MADS BOX PROTEIN FLOWERING LOCUS F; REDUCED STEM BRANCHING 6; RSB6; T31P16.130; T31P16_130; FLC
Target Background
MADS-box protein encoded by FLOWERING LOCUS C - transcription factor that functions as a repressor of floral transition and contributes to temperature compensation of the circadian clock. Expression is downregulated during cold treatment. Vernalization, FRI and the autonomous pathway all influence the state of FLC chromatin. Both maternal and paternal alleles are reset by vernalization, but their earliest activation differs in timing and location. Histone H3 trimethylation at lysine 4 and histone acetylation are associated with active FLC expression, whereas histone deacetylation and histone H3 dimethylation at lysines 9 and 27 are involved in FLC repression. Expression is also repressed by two small RNAs (30- and 24-nt) complementary to the FLC sense strand 3? to the polyA site. The small RNAs are most likely derived from an antisense transcript of FLC. Interacts with SOC1 and FT chromatin in vivo. Member of a protein complex.
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