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The antibody against DBP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human DBP (NP_001343.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against DBP was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human DBP (NP_001343.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of SK-BR-3, using DBP Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
| Cat.No | ADA-05173A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | DBP |
| Target Synonyms | DABP; taxREB302; DBP | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | SK-BR-3 | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human DBP (NP_001343.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q10586 | Immunogen Sequence |
Uniprot Id
Q10586
Target Species
Human
Target Name
DBP
Target Full Name
D site-binding protein
Target Function
This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
Target Subcellular Location
Nucleus.
Target Protein Families
BZIP family, PAR subfamily
Target Tissue Specificity
Ubiquitously expressed. Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.
Target Synonyms
Albumin D box-binding protein; Albumin D-element-binding protein; D site-binding protein; DBP; DBP_HUMAN; Tax-responsive enhancer element-binding protein 302; TAXREB302; TRANSCRIPTION FACTOR DBP
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