Rabbit anti-Mouse CD146 Monoclonal Antibody

The antibody against CD146 was raised in Rabbit using the recombinant Protein corresponding to a sequence within amino acids 24-563 of mouse CD146 (NP_075548.2) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, FC, ELISA.

ADA-15485A

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Western blot analysis of various lysates, using CD146 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Specifications

Cat.No	ADA-15485A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD146
Target Synonyms	CD146; CD149; Muc18; s-endo; 1-gicerin; s-gicerin	Form	Liquid
Species Reactivity	Human, Mouse, Rat	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	bEnd.3, Mouse skin, Rat lung, Rat uterus	Application	ELISA, WB, FC, IHC-P

Immunogen Information

Immunogen Description	Recombinant Protein corresponding to a sequence within amino acids 24-563 of mouse CD146 (NP_075548.2).	Target Species	Mouse
Uniprot ID	Q8R2Y2	Immunogen Sequence

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