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The antibody against SPY was raised in rabbit using the Recombinant Escherichia coli (strain K12) SPY protein (52-147aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
The antibody against SPY was raised in rabbit using the Recombinant Escherichia coli (strain K12) SPY protein (52-147aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein G Purified. This antibody has been validated on ELISA, WB.
$299.00
Western Blot | Positive WB detected in Recombinant protein | All lanes: SPY antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 26.8 kDa | Observed band size: 33 kDa
| Cat.No | ADC-55330A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SPY |
| Target Synonyms | Periplasmic chaperone Spy (Spheroplast protein Y) spy b1743 JW1732 | Form | Liquid |
| Species Reactivity | Escherichia coli (strain K12) | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Escherichia coli (strain K12) SPY protein (52-147aa) | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P77754 |
Uniprot Id
P77754
Target Species
E.coli
Target Name
SPY
Target Function
An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface. Substrate protein folds while it is bound to chaperone. Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity. Overexpression enhances the stability of otherwise unstable periplasmic proteins.
Target Subcellular Location
Periplasm.
Target Protein Families
CpxP/Spy family
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