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Cloning the gene encoding the Human HPD protein (2-393aa) into a plasmid vector leads to the formation of recombinant plasmid. The resulting recombination plasmid is transformed into e.coli cells. e.coli cells containing the recombinant plasmid survive in the presence of a specific antibiotic and are selected to be cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, the recombinant Human HPD protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human HPD protein, demonstrating a purity exceeding 90%.
Cloning the gene encoding the Human HPD protein (2-393aa) into a plasmid vector leads to the formation of recombinant plasmid. The resulting recombination plasmid is transformed into e.coli cells. e.coli cells containing the recombinant plasmid survive in the presence of a specific antibiotic and are selected to be cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, the recombinant Human HPD protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human HPD protein, demonstrating a purity exceeding 90%.
| Cat.No | ACP04305 | Target Name | HPD |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-393aa | Mol Weight | 48.8kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P32754 |
|---|
Uniprot Id
P32754
Target Species
Human
Target Name
HPD
Target Full Name
4-hydroxyphenylpyruvate dioxygenase
Target Function
Key enzyme in the degradation of tyrosine.
Target Involvement
Tyrosinemia 3 (TYRSN3); Hawkinsinuria (HAWK)
Target Protein Families
4HPPD family
Target Research Area
Metabolism
Target Synonyms
4 HPPD; 4 hydroxyphenylpyruvate dioxygenase; 4 hydroxyphenylpyruvic acid oxidase; 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; GLOD3 ; glyoxalase domain containing 3; HPD; HPPD_HUMAN; HPPDase; PPD
Target Background
The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.
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