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The plasmid vector incorporates the gene encoding the Human RECQL protein (1-649aa), generating recombinant plasmid, which is then introduced into baculovirus cells. Selection of positive baculovirus cells is based on their capacity to withstand a particular antibiotic. Subsequently, the baculovirus cells containing the recombinant plasmid are cultivated under conditions that facilitate the expression of the Human RECQL gene. The protein carries a N-terminal 10xHis tag. Following expression, affinity purification is employed to isolate and purify the recombinant Human RECQL protein from the cell lysate. Denaturing SDS-PAGE is used to resolve the resulting recombinant protein, enabling an estimation of its purity, exceeding 85%.
The plasmid vector incorporates the gene encoding the Human RECQL protein (1-649aa), generating recombinant plasmid, which is then introduced into baculovirus cells. Selection of positive baculovirus cells is based on their capacity to withstand a particular antibiotic. Subsequently, the baculovirus cells containing the recombinant plasmid are cultivated under conditions that facilitate the expression of the Human RECQL gene. The protein carries a N-terminal 10xHis tag. Following expression, affinity purification is employed to isolate and purify the recombinant Human RECQL protein from the cell lysate. Denaturing SDS-PAGE is used to resolve the resulting recombinant protein, enabling an estimation of its purity, exceeding 85%.
| Cat.No | ACP02318 | Target Name | RECQL |
|---|---|---|---|
| Target Synonyms | RECQL; RECQ1; RECQL1; ATP-dependent DNA helicase Q1; EC 3.6.4.12; DNA helicase; RecQ-like type 1; RecQ1; DNA-dependent ATPase Q1; RecQ protein-like 1 | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 1-649aa |
| Mol Weight | 76.0 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P46063 |
|---|
Uniprot Id
P46063
Target Species
Human
Target Name
RECQL
Target Full Name
ATP-dependent DNA helicase Q1
Target Function
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Target Subcellular Location
Nucleus.
Target Protein Families
Helicase family, RecQ subfamily
Target Tissue Specificity
High expression in heart, lung, skeletal muscle and kidney, low expression in brain.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
RECQL; RECQ1; RECQL1; ATP-dependent DNA helicase Q1; EC 3.6.4.12; DNA helicase; RecQ-like type 1; RecQ1; DNA-dependent ATPase Q1; RecQ protein-like 1
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