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The expression of the recombinant Human CXCL2 protein entails introducing a DNA expression vector containing the gene for the desired protein (35-107aa) into e.coli cells through transformation. Subsequently, the cells are cultured to facilitate the expression of the intended protein. The protein features a N-terminal 10xHis-GST tag and C-terminal Myc tag fusion. The resulting recombinant Human CXCL2 protein is then gathered and purified from the cell lysate using affinity purification. Its purity, determined by SDS-PAGE, exceeds 85%.
The expression of the recombinant Human CXCL2 protein entails introducing a DNA expression vector containing the gene for the desired protein (35-107aa) into e.coli cells through transformation. Subsequently, the cells are cultured to facilitate the expression of the intended protein. The protein features a N-terminal 10xHis-GST tag and C-terminal Myc tag fusion. The resulting recombinant Human CXCL2 protein is then gathered and purified from the cell lysate using affinity purification. Its purity, determined by SDS-PAGE, exceeds 85%.
| Cat.No | ACP01884 | Target Name | CXCL2 |
|---|---|---|---|
| Target Synonyms | Growth-regulated protein beta;Gro-beta;Macrophage inflammatory protein 2-alpha;MIP2-alpha | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 35-107aa |
| Mol Weight | 44.5 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P19875 |
|---|
Uniprot Id
P19875
Target Species
Human
Target Name
CXCL2
Target Full Name
C-X-C motif chemokine 2
Target Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Target Subcellular Location
Secreted.
Target Protein Families
Intercrine alpha (chemokine CxC) family
Target Research Area
Immunology
Target Synonyms
C-X-C motif chemokine 2; Chemokine (C X C motif) ligand 2; Chemokine; CXC motif; ligand 2; CINC 2a; CINC2a; CINC3; CXC chemokine; CXCL 2; Cxcl2; CXCL2_HUMAN; Cytokine-induced neutrophil chemoattractant 3; GRO 2; GRO b; GRO protein; beta; Gro-beta; GRO-beta(5-73); GRO-beta-T; GRO2; GRO2 oncogene; GROb; GRObeta; Growth regulated protein beta; Growth-regulated protein beta; GROX; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2 alpha; Macrophage inflammatory protein 2; Macrophage inflammatory protein 2-alpha; Melanoma growth stimulatory activity beta; MGSA b; MGSA beta; MIP 2; MIP 2a; MIP2 alpha; MIP2; MIP2-alpha; MIP2A; MIP2alpha; SB-251353; SCYB 2; Scyb; SCYB2; Small inducible cytokine subfamily B; member 2
Target Background
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation.
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