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The region for expressing recombinant Human COX5A contains amino acids 42-150. The calculated molecular weight for this COX5A protein is 39.5 kDa. Expression of this COX5A protein is conducted in e.coli. The N-terminal GST tag was fused into the coding gene segment of COX5A, making it easier to detect and purify the COX5A recombinant protein in the later stages of expression and purification.The human cytochrome c oxidase subunit 5A, mitochondrial (COX5A), is a crucial component of cytochrome c oxidase (complex IV) in the mitochondrial respiratory chain. COX5A is responsible for facilitating the transfer of electrons during oxidative phosphorylation. COX5A plays a pivotal role in the final step of the electron transport chain, where electrons are transferred to molecular oxygen, producing water. This process is essential for ATP synthesis and cellular energy production. Research on COX5A focuses on understanding its role in mitochondrial function, energy metabolism, and its implications in various diseases related to mitochondrial dysfunction.
The region for expressing recombinant Human COX5A contains amino acids 42-150. The calculated molecular weight for this COX5A protein is 39.5 kDa. Expression of this COX5A protein is conducted in e.coli. The N-terminal GST tag was fused into the coding gene segment of COX5A, making it easier to detect and purify the COX5A recombinant protein in the later stages of expression and purification.The human cytochrome c oxidase subunit 5A, mitochondrial (COX5A), is a crucial component of cytochrome c oxidase (complex IV) in the mitochondrial respiratory chain. COX5A is responsible for facilitating the transfer of electrons during oxidative phosphorylation. COX5A plays a pivotal role in the final step of the electron transport chain, where electrons are transferred to molecular oxygen, producing water. This process is essential for ATP synthesis and cellular energy production. Research on COX5A focuses on understanding its role in mitochondrial function, energy metabolism, and its implications in various diseases related to mitochondrial dysfunction.
| Cat.No | ACP03077 | Target Name | COX5A |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 42-150aa | Mol Weight | 39.5kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P20674 |
|---|
Uniprot Id
P20674
Target Species
Human
Target Name
COX5A
Target Full Name
Cytochrome c oxidase subunit 5A, mitochondrial
Target Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Target Involvement
Mitochondrial complex IV deficiency is a rare condition caused by mutation in COX5A that lead to pulmonary arterial hypertension (PAH), failure to thrive and lactic acidemia.
Target Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Target Protein Families
Cytochrome c oxidase subunit 5A family
Target Research Area
Metabolism
Target Synonyms
COX; COX VA; COX5A; COX5A_HUMAN; Cytochrome c oxidase polypeptide Va; Cytochrome c oxidase polypeptide; mitochondrial; Cytochrome c oxidase subunit 5A; Cytochrome c oxidase subunit 5A; mitochondrial; Cytochrome c oxidase subunit Va; mitochondrial; Mitochondrial cytochrome c oxidase subunit Va; VA
Target Background
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
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