Recombinant Human Estradiol 17-beta-dehydrogenase 8 (HSD17B8)

Uniprot Id

Q92506

Target Species

Human

Target Name

HSD17B8

Target Full Name

(3R)-3-hydroxyacyl-CoA dehydrogenase

Target Function

Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function. Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters. NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol.

Target Subcellular Location

Mitochondrion matrix.

Target Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Target Tissue Specificity

Widely expressed, particularly abundant in prostate, placenta and kidney. Expressed at protein level in various tissues like brain, cerebellum, heart, lung, kidney, ovary, testis, adrenals and prostate.

Target Synonyms

17 beta HSD 8; 17 beta hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-hydroxysteroid dehydrogenase VIII; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-acyl-carrier protein reductase, E. coli, homolog of; Beta ketoacyl [acyl carrier protein] reductase like; D6S2245E; DHB8_HUMAN; dJ1033B10.9; Estradiol 17 beta dehydrogenase 8; Estradiol 17-beta-dehydrogenase 8; Estrogen 17 oxidoreductase; FABG; FabG, E. coli, homolog of; FabG-like; FABGL; H2 KE6; H2-KE6; HKE6; HSD17B8; Hydroxysteroid (17 beta) dehydrogenase 8; Ke-6; KE6; KE6, mouse homolog of; Protein Ke6; Really interesting new gene 2 protein; RING2; SDR30C1; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17 beta dehydrogenase 8; Testosterone 17-beta-dehydrogenase 8

Target Background

In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.