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The gene encoding the Human GLP1R protein (24-145aa) is inserted into a plasmid vector to form the recombinant plasmid, which is introduced into baculovirus cells. baculovirus cells that have successfully taken up the recombinant plasmid can be selected based on their ability to survive in the presence of a specific antibiotic. The recombinant plasmid-containing baculovirus cells are cultured under conditions that promote the expression of the interested gene. The protein is fused with a N-terminal 10xHis tag. After expression, the recombinant Human GLP1R protein is isolated and purified from the cell lysate through affinity purification. The resulting recombinant Human GLP1R protein is resolved by denaturing SDS-PAGE, allowing its purity to be estimated, greater than 85%.
The gene encoding the Human GLP1R protein (24-145aa) is inserted into a plasmid vector to form the recombinant plasmid, which is introduced into baculovirus cells. baculovirus cells that have successfully taken up the recombinant plasmid can be selected based on their ability to survive in the presence of a specific antibiotic. The recombinant plasmid-containing baculovirus cells are cultured under conditions that promote the expression of the interested gene. The protein is fused with a N-terminal 10xHis tag. After expression, the recombinant Human GLP1R protein is isolated and purified from the cell lysate through affinity purification. The resulting recombinant Human GLP1R protein is resolved by denaturing SDS-PAGE, allowing its purity to be estimated, greater than 85%.
| Cat.No | ACP02438 | Target Name | Glp1r |
|---|---|---|---|
| Target Synonyms | GLP1R; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Expression Range | 24-145aa |
| Mol Weight | 16.8kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P43220 |
|---|
Uniprot Id
P43220
Target Species
Human
Target Name
GLP1R
Target Full Name
Glucagon-like peptide 1 receptor
Target Function
G-protein coupled receptor for glucagon-like peptide 1 (GLP-1). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels. Plays a role in regulating insulin secretion in response to GLP-1.
Target Subcellular Location
Cell membrane; Multi-pass membrane protein.
Target Protein Families
G-protein coupled receptor 2 family
Target Research Area
Neuroscience, Cardiovascular
Target Synonyms
GLP1R; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R
Target Background
This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants.
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