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In the E. coli expression system, the recombinant human glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2), is synthesized by incorporating the gene fragment encoding the full-length mature human GPD2 protein (43-727aa) into a suitable expression vector. The expression vector includes an N-terminal 10xHis-tag and a C-terminal Myc-tag downstream of the gene fragment to facilitate the purification and detection of the recombinant GPD2 protein. The constructed recombinant plasmid is then introduced into E. coli host cells via transformation, and selection is performed using appropriate markers to ensure the presence of the recombinant plasmid. The transformed E. coli cells are cultured in a growth medium optimized for protein expression, and induction of protein production is achieved by adding IPTG as an inducer. Following a suitable incubation period, the cells are harvested and lysed, releasing the intracellular contents containing the expressed recombinant GPD2 protein. The purification process allows for the isolation of the recombinant protein, which exhibits a high purity level of up to 90% as determined by SDS-PAGE analysis.
In the E. coli expression system, the recombinant human glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2), is synthesized by incorporating the gene fragment encoding the full-length mature human GPD2 protein (43-727aa) into a suitable expression vector. The expression vector includes an N-terminal 10xHis-tag and a C-terminal Myc-tag downstream of the gene fragment to facilitate the purification and detection of the recombinant GPD2 protein. The constructed recombinant plasmid is then introduced into E. coli host cells via transformation, and selection is performed using appropriate markers to ensure the presence of the recombinant plasmid. The transformed E. coli cells are cultured in a growth medium optimized for protein expression, and induction of protein production is achieved by adding IPTG as an inducer. Following a suitable incubation period, the cells are harvested and lysed, releasing the intracellular contents containing the expressed recombinant GPD2 protein. The purification process allows for the isolation of the recombinant protein, which exhibits a high purity level of up to 90% as determined by SDS-PAGE analysis.
| Cat.No | ACP01220 | Target Name | GPD2 |
|---|---|---|---|
| Target Synonyms | (GPD-M)(GPDH-M)(mitohondrial glycerophosphate dehydrogenase gene)(mGDH)(mtGPD) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 43-727aa |
| Mol Weight | 81.4 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P43304 |
|---|
Uniprot Id
P43304
Target Species
Human
Target Name
GPD2
Target Full Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Target Function
Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.
Target Subcellular Location
Mitochondrion.
Target Protein Families
FAD-dependent glycerol-3-phosphate dehydrogenase family
Target Research Area
Others
Target Synonyms
GDH2; Glycerol 3 phosphate dehydrogenase 2 (mitochondrial); Glycerol 3 phosphate dehydrogenase 2; Glycerol 3 phosphate dehydrogenase mitochondrial; Glycerol-3-phosphate dehydrogenase; GPD 2; GPD M; GPD-M; gpd2; GPDH M; GPDH-M; GPDM; GPDM_HUMAN; mGPDH; mitochondrial; Mitochondrial glycerophosphate dehydrogenase; mtGPD; OTTHUMP00000162788; OTTHUMP00000204452; OTTHUMP00000204453; OTTHUMP00000204458
Target Background
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
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