Recombinant Human Insulin growth factor-like family member 1 (IGFL1)

The N-terminal 10xHis tag and C-terminal Myc tag was fused to the gene fragment corresponding to the 25-110aa of the human IGFL1 protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant human IGFL1 protein. Its purity is higher than 85%. This recombinant human IGFL1 protein showed an apparent molecular weight of about 19 kDa under SDS-PAGE condition.IGFL1 is gene encoding protein named Insulin growth factor-like family member 1. This gene belongs to IGFL family which play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth. The protein encoded by IGFL1 gene is synthesized as a precursor protein and is proteolytically cleaved to form a secreted mature peptide. This protein can be detected in ovary and spinal cord. Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member (IGFLR1).