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The recombinant Human MBD2 was expressed with the amino acid range of 143-411. The theoretical molecular weight of the MBD2 protein is 45.2 kDa. This protein is generated in a e.coli-based system. The MBD2 coding gene included the N-terminal 6xHis-KSI tag, which simplifies the detection and purification processes of the recombinant MBD2 protein in following stages of expression and purification.Methyl-CpG-binding domain protein 2 (MBD2) is a key epigenetic regulator that plays a crucial role in interpreting DNA methylation patterns in the genome. As a member of the MBD family, MBD2 specifically recognizes and binds to methylated CpG dinucleotides, influencing chromatin structure and gene expression. MBD2 is involved in gene silencing and transcriptional regulation by recruiting chromatin remodeling complexes and histone deacetylases to methylated DNA regions. It is implicated in various cellular processes, including embryonic development, genomic imprinting, and X-chromosome inactivation. Furthermore, MBD2 has been studied in the context of cancer, where alterations in DNA methylation patterns contribute to aberrant gene expression. Understanding the intricate functions of MBD2 provides insights into epigenetic mechanisms and their impact on cellular processes and disease.
The recombinant Human MBD2 was expressed with the amino acid range of 143-411. The theoretical molecular weight of the MBD2 protein is 45.2 kDa. This protein is generated in a e.coli-based system. The MBD2 coding gene included the N-terminal 6xHis-KSI tag, which simplifies the detection and purification processes of the recombinant MBD2 protein in following stages of expression and purification.Methyl-CpG-binding domain protein 2 (MBD2) is a key epigenetic regulator that plays a crucial role in interpreting DNA methylation patterns in the genome. As a member of the MBD family, MBD2 specifically recognizes and binds to methylated CpG dinucleotides, influencing chromatin structure and gene expression. MBD2 is involved in gene silencing and transcriptional regulation by recruiting chromatin remodeling complexes and histone deacetylases to methylated DNA regions. It is implicated in various cellular processes, including embryonic development, genomic imprinting, and X-chromosome inactivation. Furthermore, MBD2 has been studied in the context of cancer, where alterations in DNA methylation patterns contribute to aberrant gene expression. Understanding the intricate functions of MBD2 provides insights into epigenetic mechanisms and their impact on cellular processes and disease.
| Cat.No | ACP01708 | Target Name | MBD2 |
|---|---|---|---|
| Target Synonyms | Demethylase;DMTase;Methyl-CpG-binding protein MBD2 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 143-411aa |
| Mol Weight | 45.2 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9UBB5 |
|---|
Uniprot Id
Q9UBB5
Target Species
Human
Target Name
MBD2
Target Full Name
Methyl-CpG-binding domain protein 2
Target Function
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Target Subcellular Location
Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase.
Target Tissue Specificity
Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
Demethylase; DMTase; MBD 2; Mbd2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41
Target Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
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