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The pipeline for the recombinant Human MAP3K14 protein production in e.coli cells consists of the following consecutive steps: construction of the expression vector encoding the Human MAP3K14 protein (400-655aa), its transformation into e.coli cells, high-density cultivation and induction of the positive cells, cell lysis, and expression analysis. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The resulting recombinant Human MAP3K14 protein is purified from the cell lysate through affinity purification, and its purity is greater than 85% determined by the SDS-PAGE.
The pipeline for the recombinant Human MAP3K14 protein production in e.coli cells consists of the following consecutive steps: construction of the expression vector encoding the Human MAP3K14 protein (400-655aa), its transformation into e.coli cells, high-density cultivation and induction of the positive cells, cell lysis, and expression analysis. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The resulting recombinant Human MAP3K14 protein is purified from the cell lysate through affinity purification, and its purity is greater than 85% determined by the SDS-PAGE.
| Cat.No | ACP01815 | Target Name | MAP3K14 |
|---|---|---|---|
| Target Synonyms | Serine/threonine-protein kinase NIK NF-kappa-beta-inducing kinase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 400-655aa |
| Mol Weight | 35.4 kDa | Protein Length | Partial |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q99558 |
|---|
Uniprot Id
Q99558
Target Species
Human
Target Name
MAP3K14
Target Full Name
Mitogen-activated protein kinase kinase kinase 14
Target Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Target Subcellular Location
Cytoplasm.
Target Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Target Tissue Specificity
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Target Research Area
Biochemicals
Target Synonyms
MAP3K14; FTDCR1B; HS; HsNIK; M3K14_HUMAN; Map3k14; Mitogen activated protein kinase kinase kinase 14; Mitogen-activated protein kinase kinase kinase 14; NF kappa beta inducing kinase; NF-kappa-beta-inducing kinase; NIK; Serine/threonine-protein kinase NIK
Target Background
This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
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