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The generation of the recombinant Human NOTUM protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (20-496aa). After transfection, the cells are cultured to express the desired protein. The protein is fused with a N-terminal 6xHis tag. The recombinant Human NOTUM protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 85%, as determined by SDS-PAGE.
The generation of the recombinant Human NOTUM protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (20-496aa). After transfection, the cells are cultured to express the desired protein. The protein is fused with a N-terminal 6xHis tag. The recombinant Human NOTUM protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 85%, as determined by SDS-PAGE.
| Cat.No | ACP01337 | Target Name | NOTUM |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 20-496aa | Mol Weight | 57.8 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q6P988 |
|---|
Uniprot Id
Q6P988
Target Species
Human
Target Name
NOTUM
Target Full Name
Palmitoleoyl-protein carboxylesterase NOTUM
Target Function
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
Target Subcellular Location
Secreted.
Target Protein Families
Pectinacetylesterase family, Notum subfamily
Target Tissue Specificity
Rarely expressed in adult normal tissues.
Target Research Area
Neuroscience
Target Synonyms
NOTUM; notum pectinacetylesterase homolog (Drosophila) ; Notum pectinacetylesterase homolog; NOTUM_HUMAN; Protein notum homolog
Target Background
Enables palmitoleyl hydrolase activity. Involved in negative regulation of Wnt signaling pathway and protein depalmitoleylation. Acts upstream of or within bone development and regulation of bone mineralization. Predicted to be located in endoplasmic reticulum lumen and extracellular region.
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