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The process for recombinant Human SRP72 protein synthesis in e.coli cells includes recombining the DNA fragment that encodes the Human SRP72 protein (2-671aa) into expression vector, transforming the recombinant vector into e.coli cells, screening positive cells, cultivating and inducing cells, conducting cell lysis, and performing expression analysis. The resulting recombinant Human SRP72 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85% as determined by SDS-PAGE.
The process for recombinant Human SRP72 protein synthesis in e.coli cells includes recombining the DNA fragment that encodes the Human SRP72 protein (2-671aa) into expression vector, transforming the recombinant vector into e.coli cells, screening positive cells, cultivating and inducing cells, conducting cell lysis, and performing expression analysis. The resulting recombinant Human SRP72 protein is purified from the cell lysate using affinity purification, with its purity exceeding 85% as determined by SDS-PAGE.
| Cat.No | ACP04965 | Target Name | SRP72 |
|---|---|---|---|
| Target Synonyms | BMFF; BMFS1; Epididymis luminal protein 103; HEL103; Signal recognition particle 72 kDa protein; Signal recognition particle 72kDa; Signal recognition particle subunit SRP72; SRP72; SRP72_HUMAN | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-671aa |
| Mol Weight | 101.1 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O76094 |
|---|
Uniprot Id
O76094
Target Species
Human
Target Name
SRP72
Target Full Name
Signal recognition particle subunit SRP72
Target Function
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Target Involvement
Bone marrow failure syndrome 1 (BMFS1)
Target Subcellular Location
Cytoplasm. Endoplasmic reticulum.
Target Protein Families
SRP72 family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
BMFF; BMFS1; Epididymis luminal protein 103; HEL103; Signal recognition particle 72 kDa protein; Signal recognition particle 72kDa; Signal recognition particle subunit SRP72; SRP72; SRP72_HUMAN
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