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The generation of the recombinant Human TCAP protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human TCAP protein (1-167aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is employed to isolate and purify the recombinant TCAP protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human TCAP protein, revealing a purity level exceeding 90%.
The generation of the recombinant Human TCAP protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human TCAP protein (1-167aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is employed to isolate and purify the recombinant TCAP protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human TCAP protein, revealing a purity level exceeding 90%.
| Cat.No | ACP03460 | Target Name | TCAP |
|---|---|---|---|
| Target Synonyms | 19 kDa sarcomeric protein; CMD1N; CMH25; LGMD2G; Limb girdle muscular dystrophy 2G (autosomal recessive); T-cap; Tcap; TELE; Telethonin; TELT_HUMAN; Titin cap protein; Titin-cap | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-167aa |
| Mol Weight | 46.1kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O15273 |
|---|
Uniprot Id
O15273
Target Species
Human
Target Name
TCAP
Target Full Name
Telethonin
Target Function
Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.
Target Involvement
Cardiomyopathy, familial hypertrophic 25 (CMH25); Limb-girdle muscular dystrophy 2G (LGMD2G)
Target Subcellular Location
Cytoplasm, myofibril, sarcomere.
Target Tissue Specificity
Heart and skeletal muscle.
Target Research Area
Cardiovascular
Target Synonyms
19 kDa sarcomeric protein; CMD1N; CMH25; LGMD2G; Limb girdle muscular dystrophy 2G (autosomal recessive); T-cap; Tcap; TELE; Telethonin; TELT_HUMAN; Titin cap protein; Titin-cap
Target Background
Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.
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