Recombinant Human Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (WRN), Truncated

Recombinant Human Bifunctional 3′-5′ exonuclease/ATP-dependent helicase WRN (WRN), Truncated

Amino acids 500-946 form the expressed segment for recombinant Human WRN. This WRN protein is theoretically predicted to have a molecular weight of 58.3 kDa. Expression of this WRN protein is conducted in e.coli. The WRN gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant WRN protein during the following stages.The human bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN is a crucial protein involved in DNA metabolism and repair. It plays a central role in maintaining genome stability by participating in various DNA repair pathways, including base excision repair and double-strand break repair. Additionally, WRN is implicated in telomere maintenance, ensuring the integrity of chromosome ends. Mutations in the WRN gene are associated with Werner syndrome, a rare premature aging disorder. Ongoing research on WRN aims to elucidate its precise functions, unravel its intricate role in cellular processes, and explore potential therapeutic strategies for conditions related to DNA damage and aging.

ACP01900

Amino acids 500-946 form the expressed segment for recombinant Human WRN. This WRN protein is theoretically predicted to have a molecular weight of 58.3 kDa. Expression of this WRN protein is conducted in e.coli. The WRN gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant WRN protein during the following stages.The human bifunctional 3′-5′ exonuclease/ATP-dependent helicase WRN is a crucial protein involved in DNA metabolism and repair. It plays a central role in maintaining genome stability by participating in various DNA repair pathways, including base excision repair and double-strand break repair. Additionally, WRN is implicated in telomere maintenance, ensuring the integrity of chromosome ends. Mutations in the WRN gene are associated with Werner syndrome, a rare premature aging disorder. Ongoing research on WRN aims to elucidate its precise functions, unravel its intricate role in cellular processes, and explore potential therapeutic strategies for conditions related to DNA damage and aging.

Number

Cat.No	ACP01900	Target Name	WRN
Target Synonyms	DNA helicase, RecQ-like type 3)(RecQ3)(Exonuclease WRN)(RecQ protein-like 2)	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	500-946aa
Mol Weight	58.3 kDa	Protein Length	Partial
Purity	Greater than 85% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP01900

Target Name

WRN

Target Synonyms

DNA helicase, RecQ-like type 3)(RecQ3)(Exonuclease WRN)(RecQ protein-like 2)

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

500-946aa

Mol Weight

58.3 kDa

Protein Length

Partial

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q14191

Background Information

Uniprot Id

Q14191
Target Species

Human
Target Name

WRN
Target Full Name

Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
Target Function

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Plays a role in double-strand break repair after gamma-irradiation.
Target Involvement

Werner syndrome (WRN); Colorectal cancer (CRC)
Target Subcellular Location

Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Chromosome.
Target Protein Families

Helicase family, RecQ subfamily
Target Research Area

Epigenetics and Nuclear Signaling
Target Synonyms

DKFZp686C2056; DNA helicase; DNA helicase, RecQ like type 3; Exonuclease WRN; HGNC 12791; OTTHUMP00000225301; RecQ protein-like 2; RecQ-like type 3; RecQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome helicase; Werner syndrome protein; Werner syndrome, RecQ helicase like; WRN; WRN_HUMAN
Target Background

This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers.

Recombinant Human Bifunctional 3′-5′ exonuclease/ATP-dependent helicase WRN (WRN), Truncated

Specifications

Immunogen Information

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