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The synthesis of the recombinant plasmid containing the gene encoding the Human MAGEA10 protein (1-369aa) is the first step to produce the recombinant Human MAGEA10 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human MAGEA10 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human MAGEA10 protein, indicating a purity level exceeding 85%.
The synthesis of the recombinant plasmid containing the gene encoding the Human MAGEA10 protein (1-369aa) is the first step to produce the recombinant Human MAGEA10 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal 10xHis tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human MAGEA10 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human MAGEA10 protein, indicating a purity level exceeding 85%.
| Cat.No | ACP02321 | Target Name | MAGEA10 |
|---|---|---|---|
| Target Synonyms | MAGEA10; MAGE10Melanoma-associated antigen 10; Cancer/testis antigen 1.10; CT1.10; MAGE-10 antigen | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-369aa |
| Mol Weight | 46.3 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P43363 |
|---|
Uniprot Id
P43363
Target Species
Human
Target Name
MAGEA10
Target Full Name
Melanoma-associated antigen 10
Target Function
Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Target Subcellular Location
Nucleus.
Target Tissue Specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for spermatogonia, spermatocytes and placenta.
Target Research Area
Cancer
Target Synonyms
MAGEA10; MAGE10Melanoma-associated antigen 10; Cancer/testis antigen 1.10; CT1.10; MAGE-10 antigen
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