Recombinant Human Probable ATP-dependent RNA helicase DDX53 (DDX53)

The recombinant human DDX53 protein is a fusion protein consists of the human DDX53 protein (1-631aa) partnered with the N-terminal 10xHis tag and C-terminal Myc tag. It was produced in the E.coli. This recombinant DDX53 protein's purity is greater than 85% determined by SDS-PAGE. After electrophoresis, there is a 78 kDa protein band presented on the gel.Human Probable ATP-dependent RNA helicase DDX53 functions as an oncogene and increases the level of cyclins. Aberrant expression of DDX53 has been documented in various cancer tissues and cell lines. Helicases are implicated in most stages of the gene expression pathway, ranging from DNA replication, RNA transcription, splicing, RNA transport, ribosome biogenesis, mRNA translation, RNA storage and decay. Besides, methylation has an important role for the expression regulation of DDX53. It has been found that DDX53 confers resistance to various anti-cancer drugs. Furthermore, a study noted that DDX53 promotes cancer stem cell-like properties and autophagy. DDX53 showed a co-expression with CD133, and binding to SOX-2, a marker of cancer stemness. Threrfore, DDX53 has been regared as a novel role of DDX53 in regulating cancer stem cell-like properties. Additionally, Activation of DDX53 was observed in a variety of tumors, including gastric and endometrial cancers, as well as in hematological cancers.