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The DNA coding sequence translated into the human KRT1 protein sequence (2-644aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human KRT1 protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 85%. After electrophoresis, a 66 kDa protein band was observed on the gel.KRT1 is gene encoding a protein called Keratin, type II cytoskeletal 1 (also known as Keratin-1) in human and belongs to intermediate filament family.? The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-1 is expressed in terminally differentiating epidermis. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.
The DNA coding sequence translated into the human KRT1 protein sequence (2-644aa) was fused with the N-terminal 6xHis tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant human KRT1 protein with N-terminal 6xHis tag. The SDS-PAGE analysis determined its purity higher than 85%. After electrophoresis, a 66 kDa protein band was observed on the gel.KRT1 is gene encoding a protein called Keratin, type II cytoskeletal 1 (also known as Keratin-1) in human and belongs to intermediate filament family.? The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-1 is expressed in terminally differentiating epidermis. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.
| Cat.No | ACP02420 | Target Name | KRT1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 2-644aa | Mol Weight | 69.9 kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P04264 |
|---|
Uniprot Id
P04264
Target Species
Human
Target Name
KRT1
Target Full Name
Keratin, type II cytoskeletal 1
Target Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Target Involvement
Epidermolytic hyperkeratosis (EHK); Ichthyosis hystrix, Curth-Macklin type (IHCM); Keratoderma, palmoplantar, non-epidermolytic (NEPPK); Ichthyosis annular epidermolytic (AEI); Keratoderma, palmoplantar, striate 3 (SPPK3)
Target Subcellular Location
Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells.
Target Protein Families
Intermediate filament family
Target Tissue Specificity
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Target Research Area
Signal Transduction
Target Synonyms
67 kDa cytokeratin; CK-1; CK1; Cytokeratin-1; Cytokeratin1; EHK; EHK1; Epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; K2C1_HUMAN; Keratin; Keratin type II cytoskeletal 1; Keratin-1; Keratin1; KRT 1; Krt1; KRT1A; NEPPK; type II cytoskeletal 1; Type II keratin Kb1; Type-II keratin Kb1
Target Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
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