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The recombinant Human ANGPT2 protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human ANGPT2 protein (19-485aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the ANGPT2 protein. The protein carries a N-terminal 6xHis tag. Cell lysis is performed to gather the recombinant Human ANGPT2 protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ANGPT2 protein reaches up to 90%.
The recombinant Human ANGPT2 protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human ANGPT2 protein (19-485aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the ANGPT2 protein. The protein carries a N-terminal 6xHis tag. Cell lysis is performed to gather the recombinant Human ANGPT2 protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ANGPT2 protein reaches up to 90%.
| Cat.No | ACP04518 | Target Name | ANGPT2 |
|---|---|---|---|
| Target Synonyms | AGPT 2; Agpt2; ANG 2; ANG-2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin-2; Angiopoietin2; ANGP2_HUMAN; ANGPT 2; Angpt2; Tie2 ligand | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 19-485aa |
| Mol Weight | 57.7kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | O15123 |
|---|
Uniprot Id
O15123
Target Species
Human
Target Name
ANGPT2
Target Full Name
Angiopoietin-2
Target Function
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Target Subcellular Location
Secreted.
Target Research Area
Cardiovascular
Target Synonyms
AGPT 2; Agpt2; ANG 2; ANG-2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin-2; Angiopoietin2; ANGP2_HUMAN; ANGPT 2; Angpt2; Tie2 ligand
Target Background
This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome.
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