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The recombinant Human PADI3 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human PADI3 protein (1-664aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the PADI3 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human PADI3 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human PADI3 protein reaches over 90%.
The recombinant Human PADI3 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human PADI3 protein (1-664aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the PADI3 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human PADI3 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human PADI3 protein reaches over 90%.
| Cat.No | ACP04534 | Target Name | PADI3 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 1-664aa | Mol Weight | 76.7kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9ULW8 |
|---|
Uniprot Id
Q9ULW8
Target Species
Human
Target Name
PADI3
Target Full Name
Protein-arginine deiminase type-3
Target Function
Catalyzes the deimination of arginine residues of proteins.
Target Involvement
Uncombable hair syndrome 1 (UHS1)
Target Subcellular Location
Cytoplasm.
Target Protein Families
Protein arginine deiminase family
Target Tissue Specificity
Hair follicles, and epidermis at very low levels.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
PADI3; PADI3_HUMAN; PDI3; Peptidyl arginine deiminase; type III; Peptidylarginine deiminase III; Protein arginine deiminase type 3; Protein arginine deiminase type III; Protein-arginine deiminase type III; Protein-arginine deiminase type-3
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