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The production of this recombinant Human RASA1 protein is just like all recombinant proteins. The process involved transfecting Mammalian cell cells with DNA vector containing the template of recombinant DNA. The Mammalian cell cells containing the template were then cultured so that they could transcribe and translate the RASA1 protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. The purity is 85% determined by SDS-PAGE. RASA1 is a gene providing instructions for making a protein named Ras GTPase-activating protein 1 (also known as GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator or p120GAP). RasGAP, an inhibitory regulator of the Ras-cyclic AMP pathway, is a multifunctional molecule involved in blood vessel morphogenesis, intracellular signal transduction, negative regulation of apoptotic process and cell adhesion, regulation of actin filament polymerization and regulation of RNA metabolic process.
The production of this recombinant Human RASA1 protein is just like all recombinant proteins. The process involved transfecting Mammalian cell cells with DNA vector containing the template of recombinant DNA. The Mammalian cell cells containing the template were then cultured so that they could transcribe and translate the RASA1 protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. The purity is 85% determined by SDS-PAGE. RASA1 is a gene providing instructions for making a protein named Ras GTPase-activating protein 1 (also known as GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator or p120GAP). RasGAP, an inhibitory regulator of the Ras-cyclic AMP pathway, is a multifunctional molecule involved in blood vessel morphogenesis, intracellular signal transduction, negative regulation of apoptotic process and cell adhesion, regulation of actin filament polymerization and regulation of RNA metabolic process.
| Cat.No | ACP04964 | Target Name | RASA1 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 714-1047aa | Mol Weight | 45.6 kDa |
| Protein Length | Partial | Purity | Greater than 85% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P20936 |
|---|
Uniprot Id
P20936
Target Species
Human
Target Name
RASA1
Target Full Name
Ras GTPase-activating protein 1
Target Function
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.
Target Involvement
Capillary malformation-arteriovenous malformation (CMAVM); Parkes Weber syndrome (PKWS)
Target Subcellular Location
Cytoplasm.
Target Tissue Specificity
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
Target Research Area
Neuroscience
Target Synonyms
CM AVM; CMAVM; DKFZp434N071; GAP; GTPase activating protein; GTPase-activating protein; OTTHUMP00000222390; OTTHUMP00000222391; OTTHUMP00000222392; OTTHUMP00000222393; p120GAP; p120RASGAP; PKWS; Ras GTPase-activating protein 1; RAS p21 protein activator (GTPase activating protein) 1; Ras p21 protein activator; RASA; RASA1; RASA1_HUMAN; RasGAP; Triphosphatase activating protein
Target Background
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
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