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The recombinant antibody against PIN4 was produced using a synthesized peptide derived from human PIN4 as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IF.
The recombinant antibody against PIN4 was produced using a synthesized peptide derived from human PIN4 as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IF.
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Western Blot | Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate | All lanes: PIN4 antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14, 17, 15 kDa | Observed band size: 12-17 kDa
| Cat.No | ADC-56629A | Clonality | Monoclonal |
|---|---|---|---|
| Target Name | PIN4 | Target Synonyms | PIN4 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | Rabbit IgG | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline |
| Purification Method | Affinity-chromatography purified | Conjugate | Non-conjugated |
| Application | ELISA, IF, IHC, WB | Storage | Upon receipt |
| Immunogen Description | A synthesized peptide derived from human PIN4 | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9Y237 |
Uniprot Id
Q9Y237
Target Species
Human
Target Name
PIN4
Target Full Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
Target Function
Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.; Isoform 2 binds to double-stranded DNA.
Target Subcellular Location
[Isoform 1]: Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.; [Isoform 2]: Mitochondrion. Mitochondrion matrix. Note=Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.
Target Protein Families
PpiC/parvulin rotamase family, PIN4 subfamily
Target Tissue Specificity
Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much m
Target Synonyms
EPVH; PAR14; PAR17; hEPVH; hPar14; hPar17; PIN4
Target Background
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria.
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