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The recombinant antibody against DBF4 was produced using a synthesized peptide derived from human DBF4 as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IF.
The recombinant antibody against DBF4 was produced using a synthesized peptide derived from human DBF4 as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IF.
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Western Blot | Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate | All lanes: DBF4 antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 77, 27 kDa | Observed band size: 72-95 kDa
| Cat.No | ADC-56663A | Clonality | Monoclonal |
|---|---|---|---|
| Target Name | DBF4 | Target Synonyms | ASK DBF4A ZDBF1, DBF4, Protein DBF4 homolog A (Activator of S phase kinase) (Chiffon homolog A) (DBF4-type zinc finger-containing protein 1) |
| Form | Liquid | Species Reactivity | Human |
| Isotype | Rabbit IgG | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline |
| Purification Method | Affinity-chromatography purified | Conjugate | Non-conjugated |
| Application | ELISA, IF, WB | Storage | Upon receipt |
| Immunogen Description | A synthesized peptide derived from human DBF4 | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9UBU7 |
Uniprot Id
Q9UBU7
Target Species
Human
Target Name
DBF4
Target Full Name
Protein DBF4 homolog A
Target Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Target Subcellular Location
Nucleus.
Target Tissue Specificity
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Target Synonyms
Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4 homolog; DBF4 type zinc finger containing protein 1; DBF4 zinc finger A; DBF4 zinc finger; DBF4; S. cerevisiae; homolog of; DBF4-type zinc finger-containing protein 1; DBF4A; DBF4A_HUMAN; Dumbbell former 4; S. cerevisiae; homolog of; OTTHUMP00000161661; Protein DBF4 homolog A; ZDBF1; Zinc finger; DBF-type containing 1
Target Background
Predicted to enable protein serine/threonine kinase activator activity. Predicted to be involved in positive regulation of nuclear cell cycle DNA replication and regulation of cell cycle phase transition. Located in nuclear body.
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