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The antibody against CA5B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against CA5B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of various lysates, using CA5B Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
| Cat.No | ADA-10513A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CA5B |
| Target Synonyms | CAVB; CA-VB; CA5B | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse kidney, Rat kidney, Rat lung | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q9Y2D0 | Immunogen Sequence |
Uniprot Id
Q9Y2D0
Target Species
Human
Target Name
CA5B
Target Full Name
Carbonic anhydrase 5B, mitochondrial
Target Function
Reversible hydration of carbon dioxide.
Target Subcellular Location
Mitochondrion.
Target Protein Families
Alpha-carbonic anhydrase family
Target Tissue Specificity
Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver.
Target Synonyms
CA VB; CA-VB; CA5B; CAH5B_HUMAN; Carbonate dehydratase VB; Carbonic anhydrase 5B; Carbonic anhydrase 5B; mitochondrial; Carbonic anhydrase VB; Carbonic anhydrase VB; mitochondrial; mitochondrial
Target Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes carbonic anhydrase 5B. CA5B, and the related CA5A gene, has its expression localized in the mitochondria though CA5B has a wider tissue distribution than CA5A, which is restricted to the liver, kidneys, and skeletal muscle. A carbonic anhydrase pseudogene (CA5BP1) is adjacent to the CA5B gene and these two loci produce CA5BP1-CA5B readthrough transcripts.
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