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The antibody against NELFB was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against NELFB was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
| Cat.No | ADA-02130A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | NELFB |
| Target Synonyms | COBRA1; NELF-B; NELFB | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, Mouse kidney, 293T, LO2, Mouse brain, Mouse liver, SKOV3, U-87MG | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q8WX92 | Immunogen Sequence |
Uniprot Id
Q8WX92
Target Species
Human
Target Name
NELFB
Target Full Name
Negative elongation factor B
Target Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
Target Subcellular Location
Nucleus.
Target Protein Families
NELF-B family
Target Tissue Specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Target Research Area
Transcription
Target Synonyms
NELFB; COBRA1; KIAA1182; Negative elongation factor B; NELF-B; Cofactor of BRCA1
Target Background
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).
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