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The antibody against SIM2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_005060.1?) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against SIM2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_005060.1?) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of various lysates, using SIM2 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-10142A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SIM2 |
| Target Synonyms | SIM; bHLHe15; HMC13F06; HMC29C01; SIM2 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse kidney, HepG2, PC-3 | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_005060.1?). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE | Uniprot ID | Q14190 |
Uniprot Id
Q14190
Target Species
Human
Target Name
SIM2
Target Full Name
Single-minded homolog 2
Target Function
Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
Target Subcellular Location
Nucleus.
Target Research Area
Cancer
Target Synonyms
bHLHe15; Class E basic helix loop helix protein 15; Class E basic helix-loop-helix protein 15; MGC119447; SIM 2; SIM; Sim2; SIM2_HUMAN; Single minded homolog 2 (Drosophila); Single minded homolog 2; Single-minded homolog 2; Transcription factor SIM2
Target Background
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.
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