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The antibody against ST6GAL1/CD75 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 44-406 of human ST6GAL1/CD75 (NP_001340845.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, FC, ELISA.
The antibody against ST6GAL1/CD75 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 44-406 of human ST6GAL1/CD75 (NP_001340845.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, IF/ICC, FC, ELISA.
Western blot analysis of lysates from Hep G2 cells, using ST6GAL1/CD75 Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
| Cat.No | ADA-15541A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ST6GAL1/CD75 |
| Target Synonyms | ST6N; CDw75; SIAT1; ST6GalI; ST6GAL1/CD75 | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Hep G2 | Application | ELISA, WB, FC, IF/ICC, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 44-406 of human ST6GAL1/CD75 (NP_001340845.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P15907 | Immunogen Sequence |
Uniprot Id
P15907
Target Species
Human
Target Name
ST6GAL1
Target Full Name
Beta-galactoside alpha-2,6-sialyltransferase 1
Target Function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Target Subcellular Location
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Target Protein Families
Glycosyltransferase 29 family
Target Synonyms
ST6GAL1; SIAT1; Beta-galactoside alpha-2,6-sialyltransferase 1; Alpha 2,6-ST 1; B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1
Target Background
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.
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