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The antibody against ZNF131 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 80-240 of human ZNF131 (NP_003423.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against ZNF131 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 80-240 of human ZNF131 (NP_003423.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of 293F, using ZNF131 Rabbit pAb at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
| Cat.No | ADA-05133A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ZNF131 |
| Target Synonyms | ZBTB35; pHZ-10; ZNF131 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | 293F | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 80-240 of human ZNF131 (NP_003423.1). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | MAFRHLIEFTYTAKLMIQGEEEANDVWKAAEFLQMLEAIKALEVRNKENSAPLEENTTGKNEAKKRKIAETSNVITESLPSAESEPVEIEVEIAEGTIEVEDEGIETLEEVASAKQSVKYIQSTGSSDDSALALLADITSKYRQGDRKGQIKEDGCPSDPT | Uniprot ID | P52739 |
Uniprot Id
P52739
Target Species
Human
Target Name
ZNF131
Target Full Name
Zinc finger protein 131
Target Function
Plays a role during development and organogenesis as well as in the function of the adult central nervous system. May be involved in transcriptional regulation as a repressor of ESR1/ER-alpha signaling.
Target Subcellular Location
Nucleus. Note=Sumoylation does not affect nuclear localization.
Target Protein Families
Krueppel C2H2-type zinc-finger protein family
Target Tissue Specificity
Predominant expression is found in different brain areas such as the occipital and temporal lobe, the nucleus caudatus, hippocampus, and the cerebellum as well as in testis and thymus.
Target Synonyms
ZNF131Zinc finger protein 131
Target Background
Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription repressor activity, RNA polymerase II-specific; and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Located in intermediate filament cytoskeleton and nucleoplasm.
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