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The antibody against ply was raised in rabbit using the Recombinant Streptococcus pneumoniae serotype 2 ply protein (2-471aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
The antibody against ply was raised in rabbit using the Recombinant Streptococcus pneumoniae serotype 2 ply protein (2-471aa) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in: recombinant protein | All lanes: ply Antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 66 kDa | Observed band size: 66 kDa
| Cat.No | ADC-55271A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ply |
| Target Synonyms | ply, Pneumolysin (Thiol-activated cytolysin) | Form | Liquid |
| Species Reactivity | Streptococcus pneumoniae serotype 2 | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Affinity-chromatography purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Streptococcus pneumoniae serotype 2 ply protein (2-471aa) | Target Species | Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q04IN8 |
Uniprot Id
Q04IN8
Target Species
Streptococcus pneumoniae serotype 2
Target Name
PLY
Target Function
A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Target Subcellular Location
Secreted. Host cell membrane; Multi-pass membrane protein.
Target Protein Families
Thiol-activated cytolysin family
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