Recombinant Human 14-3-3 protein theta (YWHAQ)

Recombination of a plasmid encoding the Human YWHAQ protein (1-245aa) is the first step during the production the recombinant Human YWHAQ protein. The constructed plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected to be cultured for the induction of protein expression. The protein is equipped with a N-terminal GST tag. After expression, affinity purification is used to isolate and purify the recombinant Human YWHAQ protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human YWHAQ protein. Its purity exceeds 90%.

ACP03587

Number

Cat.No	ACP03587	Target Name	YWHAQ
Target Synonyms	theta polypeptide; Ywhaq	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	1-245aa
Mol Weight	54.8kDa	Protein Length	Full length
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP03587

Target Name

YWHAQ

Target Synonyms

theta polypeptide; Ywhaq

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-245aa

Mol Weight

54.8kDa

Protein Length

Full length

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	P27348

Background Information

Uniprot Id

P27348
Target Species

Human
Target Name

YWHAQ
Target Full Name

14-3-3 protein theta
Target Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Target Subcellular Location

Cytoplasm. Note=In neurons, axonally transported to the nerve terminals.
Target Protein Families

14-3-3 family
Target Tissue Specificity

Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression
Target Research Area

Neuroscience
Target Synonyms

14-3-3 protein T cell; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 1433T_HUMAN; IC5; Protein HS1; Protein tau; YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; Ywhaq
Target Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Recombinant Human 14-3-3 protein theta (YWHAQ)

Specifications

Immunogen Information

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