Recombinant Human 5' exonuclease Apollo (DCLRE1B)

Cat.No	ACP10974	Target Name	DCLRE1B
Target Synonyms	DCLRE1B; SNM1B5' exonuclease Apollo; EC 3.1.-.-; DNA cross-link repair 1B protein; SNM1 homolog B; SNMIB; hSNM1B	Form	Lyophilized powder
Expression System	Custom Production. Please inquire and provide the desire expression system.	Expression Range	1-532
Protein Length	Full length protein	Purity	>85% (SDS-PAGE)
Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP10974

Target Name

DCLRE1B

Target Synonyms

DCLRE1B; SNM1B5' exonuclease Apollo; EC 3.1.-.-; DNA cross-link repair 1B protein; SNM1 homolog B; SNMIB; hSNM1B

Form

Lyophilized powder

Expression System

Custom Production. Please inquire and provide the desire expression system.

Expression Range

1-532

Protein Length

Full length protein

Purity

>85% (SDS-PAGE)

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	Q9H816

Background Information

Uniprot Id

Q9H816
Target Species

Human
Target Name

DCLRE1B
Target Full Name

5' exonuclease Apollo
Target Function

5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint. Possesses beta-lactamase activity, catalyzing the hydrolysis of penicillin G and nitrocefin. Exhibits no activity towards other beta-lactam antibiotic classes including cephalosporins (cefotaxime) and carbapenems (imipenem).
Target Involvement

Hoyeraal-Hreidarsson syndrome (HHS)
Target Subcellular Location

Chromosome, telomere. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Mainly localizes to telomeres, recruited via its interaction with TERF2. During mitosis, localizes to the centrosome.
Target Protein Families

DNA repair metallo-beta-lactamase (DRMBL) family
Target Synonyms

DCLRE1B; SNM1B5' exonuclease Apollo; EC 3.1.-.-; DNA cross-link repair 1B protein; SNM1 homolog B; SNMIB; hSNM1B
Target Background

DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).

Recombinant Human 5′ exonuclease Apollo (DCLRE1B)

Specifications

Immunogen Information

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