Recombinant Human 7SK snRNA methylphosphate capping enzyme (MEPCE)

Uniprot Id

Q7L2J0

Target Species

Human

Target Name

MEPCE

Target Full Name

7SK snRNA methylphosphate capping enzyme

Target Function

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.

Target Subcellular Location

Nucleus.

Target Protein Families

Methyltransferase superfamily

Target Tissue Specificity

Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.

Target Synonyms

MEPCE; BCDIN37SK snRNA methylphosphate capping enzyme; MePCE; EC 2.1.1.-; Bicoid-interacting protein 3 homolog; Bin3 homolog

Target Background

Enables 7SK snRNA binding activity and RNA 5'-methyltransferase activity. Involved in RNA modification; positive regulation of protein localization to Cajal body; and positive regulation of snRNA transcription by RNA polymerase II. Located in nucleus. Part of 7SK snRNP.