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Insertion of the gene encoding the Human ADH4 protein (1-380aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal GST tag is attached to the protein. Following expression, the recombinant Human ADH4 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ADH4 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
Insertion of the gene encoding the Human ADH4 protein (1-380aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal GST tag is attached to the protein. Following expression, the recombinant Human ADH4 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human ADH4 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
| Cat.No | ACP03509 | Target Name | ADH4 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | E.coli |
| Expression Range | 1-380aa | Mol Weight | 67.2kDa |
| Protein Length | Full length | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P08319 |
|---|
Uniprot Id
P08319
Target Species
Human
Target Name
ADH4
Target Full Name
All-trans-retinol dehydrogenase [NAD(+)] ADH4
Target Function
Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Also catalyzes the reduction of benzoquinones.
Target Subcellular Location
Cytoplasm.
Target Protein Families
Zinc-containing alcohol dehydrogenase family, Class-II subfamily
Target Research Area
Signal Transduction
Target Synonyms
Ac1002; ADH 4; ADH-1; ADH-2; ADH2; Adh4; ADH4_HUMAN; Alcohol dehydrogenase 4 (class II) pi polypeptide; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain; Alcohol dehydrogenase pi isozyme ADH class II; Aldehyde reductase; Class II alcohol dehydrogenase 4 pi subunit
Target Background
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
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